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Dennis Zimmermann
Schliwa Lab
Schillerstrasse 42, D-80336 München
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Myosin V is known to play a major role in a number of eukaryotic cytoplasmic transport pathways, which involve the interplay between microtubule- and actin-based motors. These processes not only require good navigation skills through the actin meshwork, they also demand for a handoff mechanism between kinesin and myosin.
Recent studies1 unexpectedly showed that Myosin V in vitro is able to undergo a one-dimensional diffusive search on microtubules, which apparently resulted from an electrostatic interaction with the negatively charged tubulin E-hook.
To further investigate this intriguing interaction, we characterize the mechanochemical behavior of different Myosin V constructs on microtubules.

To accomplish this investigation, single-molecule in vitro motility and ATPase activity assays are used to better understand the role of electrostatics in this matter.

1 Ali, M. Y., Krementsova, E. B., Kennedy, G. G., Mahaffy, R., Pollard, T. D., Trybus, K. M. and Warshaw, D. M. PNAS, 104. 4332-4336. 2007.

Since 2008

PhD-thesis in the lab of Prof. Manfred Schliwa at the Institute for Cell Biology, Ludwig-Maximilians University (Munich-Germany).

2007-2008

Diploma-thesis in the lab of Prof. Manfred Schliwa at the Institute for Cell Biology, Ludwig-Maximilians University (Munich-Germany).

Topic: Expression and Characterization of Myosin V

2003-2008

Studies in Biology at the Ludwig-Maximilians University (Munich-Germany).

Degree: Diplom-Biologist

2007

Research internship at the Max-Planck-Institute for Biochemistry (Martinsried-Germany).

Lab: Prof. Reinhard Faessler

Topic: Cloning of ILK, Qik and CHIP from mouse embryonic fibroblasts and subsequent Characterization of these enzymes.

2006

Visiting Research-assistant at the Woods Hole Oceanographic Institute (WHOI) in Massachusetts, U.S.A.

Lab: PhD John Stegeman (Senior Scientist)

Topic: Set-up of a so-called Defensome Microarray targeting the developmental-dependent Cytochrome P450 gene expression of Danio rerio.

2003

Abitur at the Feodor-Lynen-Gymnasium (Planegg-Germany).

2000

High School Diploma at the Tourtellotte Memorial High School, Connecticut (U.S.A.).

Publications:

Zimmermann D, Abdel Motaal B, Voith von Voithenberg L, Schliwa M, Ökten Z (2011) Diffusion of Myosin V on Microtubules: A Fine-tuned Interaction for Which E-hooks Are Dispensable. PLoS ONE 6(9): e25473.

Zimmermann, D. (2011) Germany: Experiencing a New Age of Research and Teaching. ASCB Newsletter 34 (4): 15-17.


Posters:

Zimmermann D., Schliwa M. and Oekten Z. Myosin V scans along microtubules in an E-hook-independent fashion. Cellular Cytoskeletal Motor Proteins Meeting of the Biochemical Society 2011, Cambridge (U.K.), Abstract-No. P071.

Zimmermann D., Schliwa M. and Oekten Z. Diffusion of Myosin V along Microtubules: A Two-Step Process. Annual ASCB Meeting 2010, Philadelphia (U.S.A.), Abstract-No. 116/B164.

Zimmermann D., Schliwa, M. and Oekten, Z. Myosin V on Microtubules: An Unexpected Interaction. Annual EMBO Meeting 2010, Barcelona (Spain), Abtsract-No. B101.

Zimmermann D., Schliwa M. and Oekten Z. The Attraction of Myosin V towards Microtubules. Interact Symposium 2010, Munich, Abstract-No. 43

Zimmermann D., Schliwa M. and Oekten Z. The Attraction of Myosin V towards Microtubules. Annual DGZ Meeting 2010, Regensburg, Abstract-No.

Zimmermann D., Schliwa M. and Oekten Z. Investigating Electrostatic Interaction of Myosin V with Microtubules. ELSO-Meeting 2008, Nizza (France), Abstract-No. 142.


Talks:

"How is Networking of the Cytoskeleton Achieved?" The Chicago Cytoskeleton, Chicago (U.S.A.), 16 September 2011.


"Two Transport Systems, One Goal: How is Networking of the Cytoskeleton Achieved?" The 2011 Meeting of Nobel Laureates, Lindau (Germany), 30 June 2011.

September 2010

Predoctoral Travel Award of the American Society for Cell Biology

August 2010

Travel Fellowship of the GlaxoSmithKline Foundation


Since 2011

Member of the Biochemical Society

Since 2010

Member of the American Society for Cell Biology (ASCB)

Since 2010

e-Fellows member

2010-2011

Spokesman of the Graduate School "Protein Dynamics in Health and Disease" in the Elite Network of Bavaria (ENB).

Since 2008

Member of the Graduate School "Protein Dynamics in Health and Disease" in the Elite Network of Bavaria (ENB).

2005-2008

Elected Member of the Faculty Student-Council at the Department for Biology of the Ludwigs-Maximilians-University (Munich-Germany).

Postdoctoral Scholar in the Kovar Lab, University of Chicago
edoc.ub.uni-muenchen.de/14668/

Contact Info

Dennis Zimmermann
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Alumni
Cell Biology
Institute for Cell Biology-LMU