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Katharina Gebendorfer
Buchner Lab
Lichtenbergstr. 4, 85747 Garching
(+49-89)-289-13191
(+49-89).....

Reactive oxygen species arise continuously during aerobic metabolism and are the core of antimicrobial responses such as the host immune system and observed in the gut and mucosal barrier epithelia. In all cases, reactive oxygen species can be harmful to cells and cause oxidative stress when increasingly accumulated. Oxidative stress leads to oxidative damage of most cellular macromolecules, causes mutations, alterations in gene expression, growth arrest and culminates ultimately in cell death. Because of the bactericidal effect of reactive oxygen species, bacteria must have developed specific mechanisms to withstand oxidative stress. We use the gram-negative bacterium Escherichia coli as a model organism to study oxidative stress responses.By screening a genomic plasmid library of E. coli for clones that are resistant to HOCl, a putative transcription factor with homology to the LysR-type familiy of transcription factors was identified. The aim of the PhD project is to analyze whether the identified gene codes for a transcription factor that is regulated by oxidative stress. We want to characterize the protein by biochemical and biophysical methods and unravel the mechanism by which this transcription factor mediates oxidative stress resistance in vivo.

2006-2011

PhD studies at the Department of Biotechnology of the Technische Universitaet Muenchen in the group of Dr. Jeannette Winter which works on identification of new mechanisms of oxidative stress resistance in Escherichia coli

2007-2006

Master studies in Biochemistry at the Technische Universitaet Muenchen

Master’s Thesis at the Molecular Nutrition Unit of the Technische Univeristaet Muenchen “Cloning, expression, purification, biochemical/biophysical characterization und functional analysis of putative membrane transporters from Methanococcus jannaschii and E.coli.”

2001-2004

Bachelor studies in Biochemistry at the Technische Universitaet Muenchen

Bachelor’s Thesis at the Department of Biotechnology of the Technische Universitaet Muenchen “Identification of the substrate binding site of Hsp104 by crosslinking, enzymatic digest and mass spectrometry.”

Rudolph B, Gebendorfer KM, Buchner J and Winter J (2010) Evolution of Escherichia coli for growth at high temperatures. J Biol Chem 285, 19029-34.

Barth E1, Gora KV1, Gebendorfer KM1, Settele F, Jakob U and Winter J (2009) Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli. Microbiology 155, 1680-9.

1these authors contributed equally

Gebendorfer KM and Winter J (2008) The periplasm of E. coli - oxidative folding of recombinant proteins. In "Oxidative Folding of Cysteine-rich Peptides and Proteins", Buchner J and Moroder L (eds.). RSC Publishing, Cambridge, UK.

organization of the visit and seminar of nobel laureate Prof. Dr. Erwin Neher in October 2008 together with Julia Rohrberg

Clinical Research Associate
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Katharina Gebendorfer
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Alumni
Biochemistry